An antibiotic sensitivity test is also known as an Antibiotic Susceptibility Test. It is conducted to find out the most effective antibiotic to combat certain bacteria in the human body. The genetic material in different bacteria varies.
The basic principle of the antibiotic susceptibility testing has been used in microbiology laboratories over 80 years. Various chemical agents such as antiseptics, disinfectants, and antibiotics are employed to combat with the microbial growth.
Testing methods. Tests for antibiotic sensitivity include: Kirby-Bauer method. Small wafers containing antibiotics are placed onto a plate upon which bacteria are growing. If the bacteria are sensitive to the antibiotic, a clear ring, or zone of inhibition, is seen around the wafer indicating poor growth.
E-test (AB Biodisk, Solna, Sweden) is a commercially available test that utilizes a plastic test strip impregnated with a gradually decreasing concentration of a particular antibiotic. The strip also displays a numerical scale that corresponds to the antibiotic concentration contained therein.
ANTIBIOTIC SENSITIVITY TESTING I. OBJECTIVES • To utilize specific monitoring techniques to evaluate the susceptibility of a microbe to different antibiotics. • To distinguish the range of activity of an antibiotic. • To recognize and define advantages and limitations of two different susceptibility testing procedures. II. INTRODUCTION
Combat antimicrobial resistance with access to the latest antimicrobials for susceptibility testing on a single platform with a system that utilizes both in vitro diagnostic (IVD) and research use only (RUO), 96-well microtitre plates for broth microdilution. With over 300 antimicrobials available in extended dilution ranges, the Sensititre System allows you to consolidate confirmatory tests while meeting FDA, CLSI and EUCAST breakpoint requirements.
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